qPCRhelper: qPCR Ct Values to Expression Values

Computes normalized cycle threshold (Ct) values (delta Ct) from raw quantitative polymerase chain reaction (qPCR) Ct values and conducts test of significance using t.test(). Plots expression values based from log2(2^(-1*delta delta Ct)) across groups per gene of interest. Methods for calculation of delta delta Ct and relative expression (2^(-1*delta delta Ct)) values are described in: Livak & Schmittgen, (2001) <doi:10.1006/meth.2001.1262>.

Version: 0.1.0
Imports: dplyr (≥ 1.0.0), rstatix (≥ 0.7.2), ggpubr (≥ 0.5.0), ggplot2 (≥ 3.4.0), magrittr (≥ 2.0.3)
Suggests: knitr, rmarkdown
Published: 2023-02-23
DOI: NA
Author: Wilson Jr. Aala
Maintainer: Wilson Jr. Aala <s98107020 at gs.ncku.edu.tw>
License: GPL-3
NeedsCompilation: no
Materials: NEWS
CRAN checks: qPCRhelper results

Documentation:

Reference manual: qPCRhelper.pdf
Vignettes: qPCRhelper

Downloads:

Package source: qPCRhelper_0.1.0.tar.gz
Windows binaries: r-devel: qPCRhelper_0.1.0.zip, r-release: qPCRhelper_0.1.0.zip, r-oldrel: qPCRhelper_0.1.0.zip
macOS binaries: r-release (arm64): qPCRhelper_0.1.0.tgz, r-oldrel (arm64): qPCRhelper_0.1.0.tgz, r-release (x86_64): qPCRhelper_0.1.0.tgz, r-oldrel (x86_64): qPCRhelper_0.1.0.tgz

Linking:

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