Calculation of molecular number and brightness from fluorescence microscopy image series. The software was published in a 2016 paper. The seminal paper for the technique is Digman et al. 2008. A review of the technique was published in 2017.

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Project Status: Active – The project has reached a stable, usable state and is being actively developed. lifecycle


You can install the release version of nandb from CRAN with:


You can install the (unstable) development version of nandb from GitHub with:


I highly recommend using the release version. The dev version is just for the ultra-curious and should be thought of as unreliable.

How to use the package

See the package website at


Contributions to this package are welcome. The preferred method of contribution is through a github pull request. Feel free to contact me by creating an issue. Please note that this project is released with a Contributor Code of Conduct. By participating in this project you agree to abide by its terms.